Quantification of Solute Concentration via Spectrophotometer

by  M. M. Sorey

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Description

 

In understanding the mechanism by which electromagnetic radiation interacts with a chemical species, students will review and extend on what they have learned in previous physics and chemistry courses. Students will become familiar with how white light is absorbed or reflected by matter to produce what our eyes perceive as color. Students will be exposed to another manifestation of the quantization of light in its interaction with matter beyond the absorption and emission spectra of the Bohr model of the atom.

In the analysis of the concentration of food dyes by spectrophotometer, students will become well versed in the precise and accurate use of volumetric glassware in a fundamental analytical procedure. After becoming familiar with and accomplished in the use of volumetric pipets and flasks, students will be required to formulate a plan for making accurate and precise serial dilutions to construct a calibration curve for the determination of the concentration of a solute.

Students will learn the procedure of zeroing an instrument and the role of a blank in the calibration of an instrument. As the final assessment of the student’s acquired skill, and solution that contains an unknown concentration of analyte will assayed by the student and the student will calculate the original mass of analyte used to make the stock solution from which the unknown sample was taken.

After attaining the level of analytical skill required to conduct such an unknown determination, the student will have the option to enroll in another six week course in which the student may identify an question for research that may be addressed with spectroscopic analysis.